特殊染色试剂和染色液有效期参考资料对一些常用的染色法染液的稳定性做如下说明,配制的染液由于室内或冰箱储存的温度变化和蒸馏水的质量不同表明保质期也可能不完全相同。染色液酸性复红,Frazer-Lendrum1年酸性复红-苦味酸(Verhoeff)...
Timm’s 铜染色法固定:福尔马林固定或新鲜组织技术方法:石蜡切片或冰冻切片试剂:液体A :5%硝酸银水溶液液体B:对苯二酚2.0g, 柠檬酸5.0g, 蒸馏水100ml ,液体可以贮存在4℃暗处一个月。步骤:1.石蜡切片脱蜡至水2.0.5%硫化铵5min3.充分水洗4.用0.1N盐酸2-3min祛除铁和硫化锌液(15%三氯醋酸也 ...
DAPI staining yeast[*]1mL yeast + 0.1mL 36% formaldehyde. 1-2H/Rt [*]Wash 2x 1mL H2O (Spin at 10K, 5" ok). May leave at 4oC [*]To stain: Spind down and resuspend in 300 ul H2O. Add 700 ul 100% EtOH. Leave for 30 min/Rt. [*]Spin down, resuspend 500 ul H2O and sonicate 5 sec. [*]Spin down (10K, 5") an ...