不知那位大侠用过AMeX方法(acetone,methyl benzoate, and xylene)做过免疫组化的,我最近尝试这种方法,但切片时发现组织很脆,不知为什么?请教高手。方法是参考这篇文章 The AMeX Method:A Simplified Technique of Tissue Processing and Parafin Embedding With Improved Preservation of Antigens for Immunostaining,在NCBI上可以下载。请高手指点