I) Culture cells to 50-70% confluency. Some cells (endothelial/epithelial) may require a higher level of confluency. For suspension cells in a regular petri dish or flask, transfer enough cells suspended in medium to each well of a poly-D-lysine-coated microslide. Then Incubate microslide in CO2 incubator from 1 hour to overnight to allow cells to attach to the bottom of the slide. The incubation time varies depending on the suspension cell used. Check for cell attachment using a microscope.
Gently aspirate medium from the wells, wash the attached cells briefly with 1x PBS and dry cells.
II) Fix slides in cold acetone (-20oC) for 10 minutes, dry again and followed by 2 PBS washes.
