If your antibody works on formalin fixed tissue section, you can perfuse you animal by 10% formalin and make floating frozen section or paraffin section.
If your Ab does not work on formalin fixed section, make frozen section as normal (in OTC) and fix it by acetone. Please take care when you move out unfixed brain. 作者: dsz79 时间: 2004-5-21 16:17 标题: 请问大鼠脑和脊髓的取材固定方法