Department of Pharmacology of Kunming Medical College，Kunming650031
【Abstract】 Objective In order to solve the problem of PAP pen in the methods of sticking sample，we made the simple PAP pen of ourselves.Methods We broke a chopstick into two parts，which pointwas banded with cotton.Then the simple PAP pen was made.Afterwards，we put the pen into the neutral balsam.The pen with little neutral balsam was used to circle a ring in the simple-slice，and the ring wasabout 0.2cm away the brain of the sample.It is better not covering the sample-slice. Results The waterproof rate made by the simple PAP pen was compared98%，while the waterproof rate by other PAP pen was not above90%，so as to enhance the effect of immunohistochemistry.Conclusion This simple PAP pen is better enough to replace other PAP pen whether they are homemade or imported，and it is much cheaper.
Key words simple PAP pen neutral balsma immunohistochemistry
