我也常遇到类似的问题，顺便剪下一点供你参考。
Xylene in paraffin as a cause. I had an interesting thing
happen to me once. I worked Saturdays for a while, training a
girl at another lab in Histotechnique. The first Saturday we
cut, most sections floated to varying degrees, even things like
tonsil. The tonsil cut very nicely and seemed well processed.
Well, I was supposed to be the person in the know and I was
stumped. Took me a while but I finally figured out that they
didn't change the processors very often and there was lots of
xylene in the paraffin, and I mean lots. Apparently this was
the problem, because after changing everything and rotating on
a regular basis the problem went away. I just thought I would
throw that story in - because this experienced histotech didn't
realize that excess xylene in the paraffin could cause problems
with adhesion of sections. 作者: dingwei 时间: 2003-9-15 23:10 标题: 染色掉片的问题
