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标题: 有人做过微血管密度的免疫组化吗? [打印本页]

作者: 轻羽飞扬    时间: 2011-12-25 22:44     标题: 有人做过微血管密度的免疫组化吗?

最近因为实验需要,要做一批骨骼肌微血管密度的免疫组化切片,但以前没做过这方面的实验,想请教一下,有人做过这个实验吗?整体的流程及抗体的选择,这个是想了解到的,谢谢
作者: sy0559    时间: 2011-12-25 23:12

微血管密度我做过,用的是CD34标记的,具体的你可以去查文献,现在大家普遍用的是Weidner技术法,这个比较简单,也是公认的。
作者: shanghainese    时间: 2011-12-26 12:25

TypeNameTarget
Exclusive Endothelial cell markersCD144/VE  Cadherin
Extracellular  domain of membrane protein
Anti-VonWillibrand Factorblood vessels and  tumors
CD141surface protein
Anti-Endothelial cell (PAL-E)Vascular  endothelials but not arteries

Non-exclusive Endothelial  specific markers
CD31All  endothelials and low level on all platelets and leucocytes
CD146normal  endothelial cells, certain cancers and melanomas.
FLT-1endothelial  and hematopoietic stem cells
CD309/FLK-1endothelial  and hematopoietic stem cells
FLT-4)endothelial
cells
TIE-1embryonic  endothelial and hematopoietic stem cells
TIE-2actively growing  blood vessels
CD34
Vascular  endothelium,
granulocyte precursors,  monocytes, myeloid leukaemias.
CD54/ICAM-1
Endothelial,  dendritic, monocytes, lymphocytes
CD62P/p-Selectinactivated  platelets and vascular endothelial
CD105/EndoglinAdult  endothelial of blood vessels and some leukaemia cells





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