中华病理技术论坛 - Powered by Discuz! Board

标题: 请问SV-40病毒抗体选择哪家的染色稳定些 [打印本页]

作者: xiaoyu44003684 时间: 2013-7-16 08:40 标题: 请问SV-40病毒抗体选择哪家的染色稳定些

科室最近开展SV-40免疫组化染色，但染色效果一直不理想，我们用的抗体是SANTA的，不知有没有其他同行也用这个抗体，染色效果怎么样，不知是抗体的问题还是操作的问题。谢谢

作者: shanghainese 时间: 2013-7-16 21:59

Primary Antibody

Name: SV40 Large T Antigen Antibody

Clone: PAb 101, Mouse monoclonal

Supplier: Pharmingen

Catalog Number: 554149

Dilution: 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60 min/room temperature

Antigen Retrieval
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)
Heat/Cool Temperature: 95-100 ºC/room temperature
Heat/Cool Time: 20 minutes/20 minutes

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Counterstain
Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Results:
Staining Pattern: Nuclear

欢迎光临中华病理技术论坛 (http://bbs.zhbljs.com/bbs/)