天狼星红有F3B和4B,在某些三色法中4B被推荐染原生质(plasma)在V.G染色中代替酸性复红.F3B也在V.G法中代替酸性复红,还可用于淀粉染色,F3B证实淀粉时类似刚果红技术.用于区分胶原类型的应该是天狼星红F3B.在区分胶原成分时应该正确的选用染料.
Finally, it's important to get the right dye. Sirius red F3B is C.I. 35782 (Direct red 80). There are other "sirius red"s that are quite different. At least one that I've used a lot is OK but does not carry any C.I. designation on the label. With this kind of dye (a tetra-azo direct cotton dye) the manufacturing process necessarily generates more than one coloured product, and other compounds are added to precipitate the dye and adjust its colour intensity. Test your sirius red on sections of muscle, brain and kidney before using it for research or diagnosis. In normal kidney the glomerular basement membranes should be red but not birefringent. Every muscle fibre should be surrounded by red and birefringent collagen. I could continue, but this is already too long.
