Description: Survivin, a new inhibitor of apoptosis (IAP) protein, is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It indicates that survivin may counteract a default induction of apoptosis in G2/M phase. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis.
MoL .Vt. of Antigen: 16.5kDa
Epitope: aa 1-99
Species Reactivity: Human. Does not react with rat. Others-not tested.
Clone Designation: 4F7
Ig Isotype / Light Chain: IgG1/k:
Immunogen: Full length recombinant human
survivin protein.
Applications and Suggested Dilutions:
Ÿ Immunofluorescence
Ÿ Western Blotting (Not suitable)
Ÿ Immunohistology (Formalin/paraffm)
(Ab 2-4mg/ml for 30 min at RT; Ab-6 is better)
*[Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.]
The optimal dilution for a specific application should be determined by the investigator
Positive Control: HeLa cells. Stomach.
Cellular Localization: Cytoplasmic and nuclear
Supplied As:
200mg/ml antibody purified from the ascites fluid by
Protein G chromatography. Prepared in 10mM PBS,
pH 7.4, with 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at lmg/ml.
Storage and Stability: Antibody with sodium azide(叠氮化钠 is stable for 24 months when stored at 2-8°C. Antibody WITHOUT sodium azide is stable for 36 months when stored at below 0°C.
Key References:
1 Ambrosini G, et al. Nature Med. 3,917-921 (1997).
2. Lu, CD, et al. Cancer Res. 58, 1808-1812 (1998).
Limitations and Warranty:
Our products are intended FOR RESEARCH USE ONLY and are not approved for clinical diagnosis, drug use or therapeutic procedures. No products are to be construed as a recommendation for use in violation of any patents. We make no representations, warranties or assurances as to the accuracy or completeness of information provided on our data sheets and website. Our warranty is limited to the actual price paid for the product.
Material Safety Data:
This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling this material. The chemical, physical, and toxicological properties of this material have not been thoroughly investigated. Appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion. The material contains 0.09% sodium azide as a preservative. Although the quantity of azide is very small, appropriate care should be taken when handling this material as indicated above. The National Institute of Occupational Safety and Health has issued a bulletin citing the potential explosion hazard due to the reaction of sodium azide with copper, lead, brass, or solder in the plumbing systems. Sodium azide forms hydrazoic acid in acidic conditions and should be discarded in a large volume of running water to avoid deposits forming in metal drainage pipes.
For Research Use Only 作者: dingwei 时间: 2003-3-27 20:52 标题: 转让survivin单抗
