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标题: 新手请教：关于PBS的配制 [打印本页]

作者: july 时间: 2006-8-9 19:58 标题: 新手请教：关于PBS的配制

8gNaCl
0.2gKCl
1.44gNa2HPO4
0.24gKH2PO4
1000ml蒸馏水
这是我找到的PBS的配方，但实验室的Na2HPO4是带12H2O2的，那Na2HPO4是不是应该称3.6g呢？

另外甲醇双氧水是不是
100％甲醇 90ml
30%H2O2 10ml
这样配的

作者: 喜力 时间: 2006-8-10 12:51

你在南京什么医院?我来找你,告诉你如何配.

13951904506 我也在南京...经常在军总,鼓楼,省人民等等,,,到处"飘移"...嘿嘿...

作者: myzh 时间: 2006-8-10 19:46

我也很想知道,请知道的朋友告诉一下.或者斑主不怕麻烦说一下.我们是新手,真诚感谢.

作者: colin 时间: 2006-8-10 21:32

PBS(贮存液) 0.2M 磷酸氢二钠: 5.8 g 鳞酸二氢钠: 5.93 g  溶于100ml蒸馏水中.

      PBS(工作液)0.01 M  贮存液0.2M PB 250ML  Nacl40g加蒸馏水到5000ml.
      我们上学的时候这样配的.没有错.

作者: shanghainese 时间: 2006-8-12 23:15

Phosphate Buffered Saline (PBS)

10X PBS (0.1M PBS, pH 7.2):

   Na2HPO4 (anhydrous) -------------------------- 10.9 g

   NaH2PO4 (anhydrous) -------------------------- 3.2 g

   NaCl ------------------------------------------- 90 g

   Distilled water --------------------------------- 1000 ml

   Mix to dissolve and adjust pH to 7.2

   Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.



20X PBS (0.2M PBS, pH 7.2):

   Na2HPO4 (anhydrous) --------------------------- 21.8 g

   NaH2PO4 (anhydrous) --------------------------- 6.4 g

   NaCl ------------------------------------------- 180 g

   Distilled water ---------------------------------- 1000 ml

   Mix to dissolve and adjust pH to 7.2

   Store this solution at room temperature. Dilute 1:20 with distilled water before use and adjust pH if necessary.



10X PBS-Tween 20 (0.1M PBS, 0.5% Tween 20, pH 7.2):

      Na2HPO4 (anhydrous) -------------------------- 10.9 g

   NaH2PO4 (anhydrous) --------------------------- 3.2 g

   NaCl ------------------------------------------- 90 g

   Distilled water ---------------------------------- 1000 ml

   Mix to dissolve and adjust pH to 7.2 and then add 5 ml of Tween 20

   Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.

20X PBS-Tween 20 (0.2M PBS, 1% Tween 20, pH 7.2):

      Na2HPO4 (anhydrous) -------------------------- 21.8 g

   NaH2PO4 (anhydrous) -------------------------- 6.4 g

   NaCl ------------------------------------------ 180 g

   Distilled water --------------------------------- 1000 ml

   Mix to dissolve and adjust pH to 7.2 and then add 5 ml of Tween 20

   Store this solution at room temperature. Dilute 1:20 with distilled water before use and adjust pH if necessary.

作者: best0515 时间: 2006-8-28 19:49 标题: PBS缓冲液的配制

PBS缓冲液的配制
① 储备液
0.2M A液二水合磷酸二氢钠 6.2404g 蒸馏水 200mL
0.2M B液十二水合磷酸氢二钠 71.628g 蒸馏水 1000mL
② 0.1M PB（pH 7.2～7.4）的配制
0.2M A液 95mL
0.2M B液 405mL
蒸馏水 500mL
③ 0.1M PBS（pH 7.2～7.4）的配制
0.1M PB 1000mL
NaCl 8.5g
④ 0.01M PBS（pH 7.2～7.4）的配制
0.2M A液 47.5mL
0.2M B液 202.5mL
蒸馏水 4750mL
NaCl 45g
1N NaOH 10mL
测pH值应在7.2～7.4之间，若偏离此范围，用0.1N的HCl或NaOH调整。
0.1M的用来稀释一抗，0.01M的用于试验中漂洗。

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