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标题: 磷酸缓冲液的配制 [打印本页]

作者: shanghainese 时间: 2006-8-13 12:03 标题: 磷酸缓冲液的配制

Phosphate Buffered Saline (PBS)

10X PBS (0.1M PBS, pH 7.2):

   Na2HPO4 (anhydrous) -------------------------- 10.9 g

   NaH2PO4 (anhydrous) -------------------------- 3.2 g

   NaCl ------------------------------------------- 90 g

   Distilled water --------------------------------- 1000 ml

   Mix to dissolve and adjust pH to 7.2

   Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.



20X PBS (0.2M PBS, pH 7.2):

   Na2HPO4 (anhydrous) --------------------------- 21.8 g

   NaH2PO4 (anhydrous) --------------------------- 6.4 g

   NaCl ------------------------------------------- 180 g

   Distilled water ---------------------------------- 1000 ml

   Mix to dissolve and adjust pH to 7.2

   Store this solution at room temperature. Dilute 1:20 with distilled water before use and adjust pH if necessary.



10X PBS-Tween 20 (0.1M PBS, 0.5% Tween 20, pH 7.2):

      Na2HPO4 (anhydrous) -------------------------- 10.9 g

   NaH2PO4 (anhydrous) --------------------------- 3.2 g

   NaCl ------------------------------------------- 90 g

   Distilled water ---------------------------------- 1000 ml

   Mix to dissolve and adjust pH to 7.2 and then add 5 ml of Tween 20

   Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.

20X PBS-Tween 20 (0.2M PBS, 1% Tween 20, pH 7.2):

      Na2HPO4 (anhydrous) -------------------------- 21.8 g

   NaH2PO4 (anhydrous) -------------------------- 6.4 g

   NaCl ------------------------------------------ 180 g

   Distilled water --------------------------------- 1000 ml

   Mix to dissolve and adjust pH to 7.2 and then add 5 ml of Tween 20

   Store this solution at room temperature. Dilute 1:20 with distilled water before use and adjust pH if necessary.

作者: wsbsts-xy 时间: 2006-8-15 09:52

呵呵，这个也想看看中文版的，谢谢版主
还有就是请教，有前人确实用PBS液做抗体稀释剂，但结果也还可以，不知是否真的可以用PBS液代替专门的抗体稀释剂

作者: shanghainese 时间: 2006-8-16 11:15

没关系, 您先把它翻译过来.
我一定让您明白.

PBS可以液做抗体稀释剂, 作为临时稀释用. 但长期保存不行.
为了保持抗体的效价和降低背景, 最好用抗体稀释剂.

作者: susongxi 时间: 2006-8-28 20:44 标题: 问shanghainese

那一般用pbs 稀释的抗体可保存多长时间呢？稀释用ＰＢＳ浓度是多少呀？

作者: susongxi 时间: 2006-8-28 20:46

问shanghainese

那一般用pbs 稀释的抗体可保存多长时间呢？稀释用ＰＢＳ浓度是多少呀？

作者: shanghainese 时间: 2006-8-29 11:45

引用:

原帖由 susongxi 于 2006-8-28 12:46 发表
问shanghainese

那一般用pbs 稀释的抗体可保存多长时间呢？稀释用ＰＢＳ浓度是多少呀？

1. 不超过一周.
2. 按照上面的配方,10X的,稀释十倍, 20X的,稀释二十倍.

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