请问怎样识别外源性细胞?当把细胞移植到组织后一段时间后,取组织做H-E染,请问可以看出外源性的细胞吗?怎么看出呢? 作者: folksmile 时间: 2008-3-26 10:13 标题: tracing after injection
You can
1. Label your cells before injection, e.g. transfect a report gene into the cells and pick out the expression clones for injection; or try some alive cell dyes for short term tracing;
2. Use different genders for the donor and the acceptor and microscope the gender-related markers;
3. If the cells injected are exclusive, you could see them by the specific staining, e.g. tumor cells in normal tissue, bone cells in soft tissue, etc.
