STORING CUT SLIDES/ RETURNING TISSUE: Cut frozen sections should be stored in slide boxes wrapped air-tight in parafilm at -80 C for long-term storage and at -20 C for short-term storage (ie overnight). Tissue can be melted out of OCT by removing chuck to room temperature and holding edges of chuck (of course, with gloves) until OCT becomes gummy in consistency. With a razor blade, scrape under the tissue in OCT and place in histo-cassette. Store cassette on dry ice until transferring to -80 or to liquid nitrogen.
有的资料讲可以保存数年。 作者: liuhuaqin 时间: 2004-12-30 18:58 标题: 冰冻切片的保存
