标题:
冰冻切片固定
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作者:
kmyak
时间:
2009-1-15 01:05
标题:
冰冻切片固定
各位前辈,我做冰冻切片,新鲜组织4%多聚甲醛2h,20%蔗糖过夜后切片,用多聚赖氨酸处理过的胶片,可行否,不用切片后再用丙酮固定了吧?
作者:
grover
时间:
2009-1-17 17:20
what is the thickness of your tissue,?
you don't need to use acetone
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