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标题: 冰冻切片固定 [打印本页]

作者: kmyak    时间: 2009-1-15 01:05     标题: 冰冻切片固定

各位前辈,我做冰冻切片,新鲜组织4%多聚甲醛2h,20%蔗糖过夜后切片,用多聚赖氨酸处理过的胶片,可行否,不用切片后再用丙酮固定了吧?
作者: grover    时间: 2009-1-17 17:20

what is the thickness of your tissue,?
you don't need to use acetone




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