wowocock

最近常规的HE片子老是莫名其妙的掉片，不知道是哪个环节出了问题？ :em14:

chenrong

请你说一下你是如何操作的。
会不会天气冷了，你烘片时温度不够！《你是如何烘片的？》 :em17:  :em17:

wowocock

我们一般切完片子，放入60度的烘箱，大概两个小时左右，有时候时间会更长些

chenrong

[这个贴子最后由chenrong在 2003/12/03 08:49pm 编辑]

引用:

下面引用由wowocock在 2003/12/03 06:53pm 发表的内容：
我们一般切完片子，放入60度的烘箱，大概两个小时左右，有时候时间会更长些

如果你是暂时掉片的话，《指以前重没有过》。那你就先放在烤片机上5-10分钟，然后用电吹风快档吹5-10分钟！！！保证你不掉片。对片子无影响。光电吹风也行时间长点10-15分钟！你用放入60度的烘箱太让费时间了！
注：《个人意见》

tom

玻片是否经过处理?切片时感觉如何?脱水过度有时也容易掉片.

dingwei

主要是组织脱水处理不当。我们也用60度的烘箱，大概半个多小时多一点，片子就是用100度的开水洗也洗不下来。

wowocock

如果是脱水问题的话，是脱水过度，还是未脱干净

dingwei

过度和未脱干净，包括固定后不水洗，都会脱片。

历历

主要是组织脱水不够。

ylzhao

如果你以前没有掉片现象，是不是浸蜡缸里含二甲苯太多。
Xylene in paraffin as a cause.  I had an interesting thing
     happen to me once. I worked Saturdays for a while, training a
     girl at another lab in Histotechnique. The first Saturday we
     cut, most sections floated to varying degrees, even things like
     tonsil. The tonsil cut very nicely and seemed well processed.
     Well, I was supposed to be the person in the know and I was
     stumped. Took me a while but I finally figured out that they
     didn't change the processors very often and there was lots of
     xylene in the paraffin, and I mean lots. Apparently this was
     the problem, because after changing everything and rotating on
     a regular basis the problem went away. I just thought I would
     throw that story in - because this experienced histotech didn't
     realize that excess xylene in the paraffin could cause problems
     with adhesion of sections.