王建君

再向大家请教一个问题，我在染片中常有掉片的现象不知道问题出在那里。

sissi123

和制片过程、切片厚度、脱水快慢等都有关。

sissi123

http://www.jia-li.com/dingw/cgi-bin/topic.cgi?forum=29&topic=153&show=0
看看是否对你有帮助。

migwen

玻片和水必须干净。

ylzhao

我也常遇到类似的问题，顺便剪下一点供你参考。
Xylene in paraffin as a cause.  I had an interesting thing
     happen to me once. I worked Saturdays for a while, training a
     girl at another lab in Histotechnique. The first Saturday we
     cut, most sections floated to varying degrees, even things like
     tonsil. The tonsil cut very nicely and seemed well processed.
     Well, I was supposed to be the person in the know and I was
     stumped. Took me a while but I finally figured out that they
     didn't change the processors very often and there was lots of
     xylene in the paraffin, and I mean lots. Apparently this was
     the problem, because after changing everything and rotating on
     a regular basis the problem went away. I just thought I would
     throw that story in - because this experienced histotech didn't
     realize that excess xylene in the paraffin could cause problems
     with adhesion of sections.

dingwei

[这个贴子最后由dingwei在 2003/09/15 11:14pm 编辑]

主要是蜡块没处理好。固定，水洗，脱水等时间与步骤修改一下。福马林也很容易使切片脱片。固定后最好水洗，脱水过了，或不够，也容易脱片。低浓度酒精时间不够，也容易脱片。你把你的脱水时间写一下。

nakatp

掉片与用硬脂酸有关系吗？