Primary Antibody
Name: SV40 Large T Antigen Antibody
Clone: PAb 101, Mouse monoclonal
Supplier: Pharmingen
Catalog Number: 554149
Dilution: 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.
Incubation Time/Temp: 60 min/room temperature
Antigen Retrieval
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)
Heat/Cool Temperature: 95-100 ºC/room temperature
Heat/Cool Time: 20 minutes/20 minutes
Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods
Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature
Counterstain
Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds
Results:
Staining Pattern: Nuclear
