echo917

用tritc做二抗，一抗和二抗的稀释比都达到了最大（1：100，1：50）用山羊血亲分封闭30分钟，0.1%triton破膜。所标记的蛋白是在体内组成性表达的，可是总是抗不出来。所以想请教大家应该如何改进？(一抗用wester blot 做后没有问题)

shanghainese

Hi ech917,
This is sandiegold.  Did you receive my email?  How is your staining?  Does you primary antibody work?  Do you have any question right now?  If you have, please email me, you know my e-mail address.