shanghainese

Hi everybody,
I think most of Lab. using Harris’ Hematoxylin, it is very strong staining nuclear of cell.  So for IHC counter staining, I just stain 3 Second and washing to blue.  You also can choose Gill’s and Mayer’s Hematoxylin.  I tried using 0.1% Methyl Green or Fast red; they are very good for counterstaining.

shanghainese

I used different hematoxylin.  I know most Pathology and Histology Lab. using Harris' Hematoxylin for HE staining.  You can use it, at 1:10 dilution and for 3 second or more.  Please try different time and find optimal staining time.