引用:
原帖由 slmsisi 于 2007-10-28 21:45 发表
呵呵 谢谢了 如果我按说明书上的配1％BSA－PBS－0.05%Tween-20，具体怎么个配法？谢谢！
以下是１０倍和２０倍PBS－0.05%Tween-20的配方．
（用时稀释１０倍或２０倍）并加入１％ＢＳＡ就行了．
10X PBS-Tween 20 (0.1M PBS, 0.5% Tween 20, pH 7.2):
Na2HPO4 (anhydrous) -------------------------- 10.9 g
NaH2PO4 (anhydrous) --------------------------- 3.2 g
NaCl ------------------------------------------- 90 g
Distilled water ---------------------------------- 1000 ml
Mix to dissolve and adjust pH to 7.2 and then add 5 ml of Tween 20
Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.
20X PBS-Tween 20 (0.2M PBS, 1% Tween 20, pH 7.2):
Na2HPO4 (anhydrous) -------------------------- 21.8 g
NaH2PO4 (anhydrous) -------------------------- 6.4 g
NaCl ------------------------------------------ 180 g
Distilled water --------------------------------- 1000 ml
Mix to dissolve and adjust pH to 7.2 and then add 5 ml of Tween 20
Store this solution at room temperature. Dilute 1:20 with distilled water before use and adjust pH if necessary.
这些配方的帖子早已在本专栏里，您有空可以去找一找．
