[求助]
用试剂盒做TUNEL及相关基因蛋白免疫组化时,空白对照染色太明显(不能与阳性结果区分),请问各位我该怎么处理??
TUNEL use in situ cell death kit(POD),Roche Diagnostics Corporation,USA.基因蛋白用迈新的染色试剂盒,封闭内源性过氧化物酶后先用mouse-to-mouse block (Scytek Laboratories,USA)封闭,因为二抗是羊抗兔和鼠.
敬请各位指正错误以求改正,不胜感激!!! :em10: :em18:
