现在的问题是,我切16um的厚度,很容易卷片.而且这种厚度的切下来后,神经组织总是会垂直室管膜裂开,而且细胞比较密集的地方也会裂开.同一切片的非神经组织就没有这种现象.
不知道怎么解决?
Still have problem? Try different Tm & adjust u anti-roll bar. Check if there any bur on the blade? Clean u microtome knife thoroughly before u use it. Compare u method with that of peers'. Change it if it is necessary. Good luck.