请教Von kossa 染色的具体方法
VON KOSSA
1. Sections to water.
2. Place sections in 1% silver nitrate solution under ultra- violet light for 45 minutes.
3. Wash in distilled water.
4. Treat with 3% sodium thiosulphate for 5 minutes.
5. Wash in water.
6. Counterstain with van Gieson for 5 minutes.
7. Wash in alcohol.
8. Clear . Mount sections in DPX
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RESULTS
Mineralised bone black Osteoid red
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SOLUTIONS
VAN GIESON --1% acid fuchsin 7.5 mls
--Saturated picric acid 92.5 mls
--Distilled water 100 mls
