july

8gNaCl
0.2gKCl
1.44gNa2HPO4
0.24gKH2PO4
1000ml蒸馏水
这是我找到的PBS的配方，但实验室的Na2HPO4是带12H2O2的，那Na2HPO4是不是应该称3.6g呢？

另外甲醇双氧水是不是
100％甲醇  90ml
30%H2O2  10ml
这样配的

喜力

你在南京什么医院?我来找你,告诉你如何配.

13951904506  我也在南京...经常在军总,鼓楼,省人民等等,,,到处"飘移"...嘿嘿...

myzh

我也很想知道,请知道的朋友告诉一下.或者斑主不怕麻烦说一下.我们是新手,真诚感谢.

colin

PBS(贮存液)   0.2M 磷酸氢二钠: 5.8 g   鳞酸二氢钠: 5.93 g  溶于100ml蒸馏水中.

         PBS(工作液)0.01 M  贮存液0.2M PB 250ML  Nacl40g加蒸馏水到5000ml.
         我们上学的时候这样配的.没有错.

shanghainese

Phosphate Buffered Saline (PBS)


10X PBS (0.1M PBS, pH 7.2):

       Na2HPO4 (anhydrous) -------------------------- 10.9 g

       NaH2PO4 (anhydrous) -------------------------- 3.2 g

       NaCl ------------------------------------------- 90 g

       Distilled water --------------------------------- 1000 ml

       Mix to dissolve and adjust pH to 7.2

       Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.

  

20X PBS (0.2M PBS, pH 7.2):

       Na2HPO4 (anhydrous) --------------------------- 21.8 g

       NaH2PO4 (anhydrous) --------------------------- 6.4 g

       NaCl ------------------------------------------- 180 g

       Distilled water ---------------------------------- 1000 ml

       Mix to dissolve and adjust pH to 7.2

       Store this solution at room temperature. Dilute 1:20 with distilled water before use and adjust pH if necessary.

  

10X PBS-Tween 20 (0.1M PBS, 0.5% Tween 20, pH 7.2):

         Na2HPO4 (anhydrous) -------------------------- 10.9 g

       NaH2PO4 (anhydrous) --------------------------- 3.2 g

       NaCl ------------------------------------------- 90 g

       Distilled water ---------------------------------- 1000 ml

       Mix to dissolve and adjust pH to 7.2 and then add 5 ml of Tween 20

       Store this solution at room temperature. Dilute 1:10 with distilled water before use and adjust pH if necessary.



20X PBS-Tween 20 (0.2M PBS, 1% Tween 20, pH 7.2):

         Na2HPO4 (anhydrous) -------------------------- 21.8 g

       NaH2PO4 (anhydrous) -------------------------- 6.4 g

       NaCl ------------------------------------------ 180 g

       Distilled water --------------------------------- 1000 ml

       Mix to dissolve and adjust pH to 7.2 and then add 5 ml of Tween 20

       Store this solution at room temperature. Dilute 1:20 with distilled water before use and adjust pH if necessary.

best0515

PBS缓冲液的配制
① 储备液
0.2M A液 二水合磷酸二氢钠 6.2404g 蒸馏水 200mL
0.2M B液 十二水合磷酸氢二钠 71.628g 蒸馏水 1000mL
② 0.1M PB（pH 7.2～7.4）的配制
0.2M A液 95mL
0.2M B液 405mL
蒸馏水 500mL
③ 0.1M PBS（pH 7.2～7.4）的配制
0.1M PB 1000mL
NaCl 8.5g
④ 0.01M PBS（pH 7.2～7.4）的配制
0.2M A液 47.5mL
0.2M B液 202.5mL
蒸馏水 4750mL
NaCl 45g
1N NaOH 10mL
测pH值应在7.2～7.4之间，若偏离此范围，用0.1N的HCl或NaOH调整。
0.1M的用来稀释一抗，0.01M的用于试验中漂洗。