coldant

最近作结肠c-kit及其配体SCF免疫组化总是失败，谁有这方面的经验？

shanghainese

http://www.bdbiosciences.com/ptProduct.jsp?backLink=ptProductList.jsp&backName=Product+List&prodId=15015

coldant

I can't open it.

shanghainese

CD117 (c-kit) antibody
Clone:  YB5.B8
Format:  Purified
Isotype:  Mouse IgG1, k
W.S. No:  V C009
Target Species:  Human
Cross Reactivity:  Rabbit,Dog
Applications:  
Flow Cytometry   Recommended   APC
Flow Cytometry   Recommended   PE
Flow Cytometry   Recommended   Purified, R-PE, PE-Cy5 (BD Cy-Chrome™)

Description
Recognizes CD117 (c-kit), a 145 kDa cell-surface glycoprotein with tyrosine kinase activity. CD117 is present on hematopoietic progenitor cell subsets, tissue mast cells, and melanocytes. CD117 serves as a cytokine receptor for steel factor (SLF), also known as stem cell factor (SCF) or mast cell growth factor (MGF). The interaction of c-kit and SLF is crucial to hematopoiesis, mast cell differentiation, melanogenesis, and germ cell development. The ability of YB5.B8 antibody to block the binding of c-kit ligand is still controversial.
This antibody is suitable for staining acetone-fixed, frozen tissue sections.