闲云

请教诸位:
   P53抗体是不是对所有恶性肿瘤都应该有表达，我做了七种组织，发觉在直肠癌、乳腺癌、肺小细胞癌中表达较好，其它什么胃腺癌、肝癌、枕叶胶质瘤、肺鳞癌中是全阴性，因组织来源有限，每种只作了一个病例，不知做过这种抗体的师兄们结果如何？还望赐教！！

liuhuaqin

没有任何一种抗体具有100%的敏感性和特异性，病例一定要增加才能说明问题。

shanghainese

p53 is a nuclear phosphoprotein which acts as a tumor suppressor by providing a cell cycle checkpoint for DNA damage during S-phase. Mutations in wildtype p53 can indirectly alter the DNA binding and transcription factor activity of p53. By altering expression of genes normally regulated by p53, these mutations can result in both a loss of tumor suppressor function and a gain of oncogenic function.

闲云

我想知道的是你们做过的结果哈！

liuhuaqin

你只作了一个病例，且全为阴性，还不能判定是试剂原因、操作不对还是组织本身就是阴性。做过的结果就麻烦你自己去查一下文献。