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包埋后切片,组织的位置是空洞或一条条小碎片,是怎么原因呀?

your paraffin temperature are not high at all,infiltration temperature should be above the melting temperature 2'C-4'C,from low to high,especially lung,liver ,which is too soft ,too much air in it,the infiltration time shoud be increased to get it hard,so cutting sections will be easy.

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xylene is a universal solvent,it can mix with alcohol and paraffin,this is the keypoint of being a clearing agent,many people prefer mix xylene and alcohol together,both dehydration and clearing.

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