返回首页 | 病理技术专题 | 病理资讯 | 病理图库 | 病理技术精英 | 同行交流 | 技术会诊 | 点滴经验 | 常规切片 | 特殊染色 | 分子免疫 | 细胞园地 | 电镜技术 | 收藏本站
发新话题
打印

求助:番红O染色

求助:番红O染色

染液配制和染色步骤见英文网址:http://www.concor-cor.com/servlet/PB/show/1235150/115948en.pdf

TOP

求助:番红O染色

Safranine O/fast green for cartilage
Dewax sections, rinse in alcohol, rinse in water.
Weigert's haematoxylin - 3 minutes.
Wash in tap water.
Differentiate in acid alcohol (1% hydrochloric acid in 70% alcohol) - 15 seconds.
Wash in tap water - 3 minutes.
Stain in 0.02% aqueous fast green - 3 minutes.
Wash briefy in 1% acetic acid. Drain slide.
Stain in 0.1% aqueous safranine O - 3 minutes (maximum).
Rinse in 95% alcohol.
Dehydrate, clear and mount.
Results:  
Nuclei - black.
Cytoplasm - green.
Mucins, cartilage and mast cell granules - orange/red.

TOP

发新话题