免疫组化

echo917

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1^# 大中小发表于 2003-4-30 13:29 只看该作者

免疫组化

用tritc做二抗，一抗和二抗的稀释比都达到了****（1：100，1：50）用山羊血亲分封闭30分钟，0.1%triton破膜。所标记的蛋白是在体内组成性表达的，可是总是抗不出来。所以想请教大家应该如何改进？(一抗用wester blot 做后没有问题)

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shanghainese

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2^# 大中小发表于 2003-5-23 00:14 只看该作者

免疫组化

Hi ech917,
This is sandiegold. Did you receive my email? How is your staining? Does you primary antibody work? Do you have any question right now? If you have, please email me, you know my e-mail address.

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